Optimization in vitro conditions for plum × apricot embryo rescue and modeling some critical factors by using artificial neural networks technology

The aim of this research was to overcome abortion postzygote incompatibility, and rescuing hybrid embryos early mid-ripening plum cultivars, obtained from interspecific crossing between two cultivars (P. salicina L.) as female parents with four apricot armeniaca male parents. effects cultivar, embryo developmental stage, plant growth regulators (PGRs), cold treatment, size removing seed coats were investigated on germination at in vitro conditions. Results showed that the best conditions for rescue were: harvesting 65 d after pollination (DAP) (91.33%), using completed MS medium 0.5 mgL−1 6-benzyladenine (BA) 1 indole-3-butyric acid (IBA), Stratification 75 DAP- 4 °C 30 d. Larger testa treatment higher rates. Additionally, artificial neural networks (ANNs) used model outputs which are generation percentage (GP) complete (CFP) based three inputs including BA, IBA stage (EDS). According Multilayer 29 Perceptron (MLP), rate (100%) highest can be by culturing DAP containing 0.69 BA 0.95 IBA, or 0.94 0.98 IBA. MLP results a high correlation expected experimental values, 91.99%, 89.33% GP CFP, respectively. traditional statistical analysis Two Way ANOVA method consistent MLP. Taking whole, developed an optimal protocol may useful breeding programs.